GSM3406075: Neg_control_1_1; Mus musculus; OTHER - SRA

SRX4775077: GSM3406075: Neg_control_1_1; Mus musculus; OTHER
1 ILLUMINA (NextSeq 500) run: 22.3M spots, 3.3G bases, 1.1Gb downloads

Submitted by: NCBI (GEO)

Study: IKZF2 is required for myeloid leukemic stem cells by driving self-renewal and inhibiting the myeloid differentiation program II

PRJNA493794 • SRP162854 • All experiments • All runs

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We report that IKZF2 is required for maintaining chromatin accessibility in leukemic stem cells in myeloid leukemia. RNA seq and ATAC-seq were performed to investigate the changes in chromatin accessibility of differentially expressed genes in leukemic stem cells when IKZF2 was absent. We found that IKZF2 maintains open accessibility in self-renewal transcription factor motifs such as HOXA9 sites whereas motifs of differentiation transcription factors including C/EBPs are kept closed. Overall design: 3 Ikzf2 CUT&RUN, each 2 technical replicates; 2 Negative control, each 2 technical replicates

Sample: Neg_control_1_1

SAMN10142593 • SRS3855674 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: OTHER

Source: GENOMIC

Selection: other

Layout: PAIRED

Construction protocol: DNA was extracted using spin column. barcoded library for Illumina sequencing was generated using with Tru-Seq adapters. 60 million reads using paired end Illumina sequencing was performed.

Experiment attributes:

GEO Accession: GSM3406075

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 22.3M spots, 3.3G bases, 1.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR7939976	22,325,071	3.3G	1.1Gb	2018-10-01

ID:: 6459282

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